In the analysis on whole structural life-cycle, there are two important factors to need to be considered. One is the determination of design service life of structure, and another is design of structure based on service life. After analyzing deeply the influence factors of life-time index, it can be found that the design service life of structure not only depends on technology level, functional requirement and economic cost factors of structures, but also relate with the specific environmental conditions, using conditions and maintenance conditions of structures. So that, an analysis method of determined design service life of structure is given in this paper. For design of structure on service life, from the view of whole structural life-cycle, a probability reliability-based analysis method of structural service life design and re-design is proposed in this paper. By updating constantly design parameters, the correctness of predicted service life is improved gradually.