This paper introduces niche concept and niche overlap theory into the field of urban planning, and believe that urban niche is the extension of species niche concept. Urban provides certain resource for human beings to live and take actives, which makes different dimensions of resource, time and space in different urban's development. Regional characteristics of citiy as the manifestation of a city, which reflect different "space" position of a city in urban niche, the spatial position is multidimensional and with multiple subsystems. Regional characteristics of city mainly come from non-overlapped area of multiple subsystems in urban complex niche, the higher the proportion of non-overlapping, the more obvious features of urban regional characteristics. Therefore, this paper established a regional characteristic model of niche in city streets which based on complex ecosystem, and proposed planning principles under it, in order to emphasize regionality and create a positive ecological street.