Based on the low reversed cyclic loading experiment of a specimen of three-story prestressed fabricated concrete frame, this paper investigates the carrying capacity, the failure mode, the load-displacement hysteresis curve of frame. The research includes the seismic performances such as failure mechanism, rigidity degradation, hysteretic behavior and etc. The results indicate that the plastic hinge first appears at the ends of beam at lower story. The connections of frame are in a state of bi-direction compression and have more joint stiffness, which therefore enhances the frame lateral stiffness and improves the deformation recovery capability. The carrying capacity of the frame hasn’t serious degradation at story drifts between 1/39 and 1/64. The post-tensioned prestressed fabricated concrete frame is a “strong column weak beam” structure.