A full scale model of orthotropic deck panel was designed and fabricated referring to the common steel box girder bridges used in China. Experiment was conducted to test the behavior and fatigue performance of the orthotropic steel bridge deck under the actual truck wheel load. The loading test was conducted on the platform specially prepared. Measurement of the over-all structure and the fatigue sensitive details was made to evaluate the performance of the panel. For each gauge position, the results are presented as the form of influence lines.