This paper evaluates the shear resistance of hybrid I-beams fabricated by high performance steel and conventional steel. A number of hybrid I-beams are modeled and analyzed to determine their shear failure mechanism characteristics, considering parameters of web slenderness (hw/tw), frame action from end-stiffeners, ratio of flange width to web depth (bf/hw) and panel numbers. The analyses conclude that, in shear resistance calculation, plate beam with inter and slender webs often fail in inelastic or elastic shear buckling while ultimate shear resistance of compact webs is given by the shear strength of the material. What’s more, more rigid stiffeners provide more fixity to flange plates and increase the post-buckling resistance of plate beam. For plate beam with several panels, the shear stress at the ultimate load is similar. Finally, the I-beams with larger flange width to web depth ratio would develop larger shear strengths and then shear deformation cause formation of plastic hinges.