This paper discussed the different shopping behavior of gender in commercial building. On the base of the previous research on the physiological and shopping psychological characteristic of gender, summarize the difference of the shopping frequency, shopping time, choosing alternatives place, purchasing mode and behavior pattern of gender in commercial building, and give the dissimilarity on the requirement of the denoting system in commercial building. Through lots of the accessing records, observing shopping behavior, investigating questionnaires and testing on the physical environment, analyzes the data by using the statistical software STATISTICS and SPSS in the research. By using the physical instruments to test the indoor environments of commercial building, the paper gives the scientific attestations for the research results. The research provides the new ideas for the designing the commercial building, and make the commercial building vary humanness.