A new 3-dimensional isolation bearing (3DIB), which is combined with lead rubber bearing (LRB) and disk spring bearing (DSB), is introduced in this paper. A series of shaking table tests of the 1/2 scale fixed-base and 3DIB base-isolated model were compared to confirm the validity of the 3DIB. Results show that the 3DIB can isolate 3-dimensional earthquake energy remarkably. Large displacement of the 3DIB base-isolated system occurred on the isolation layer, and the inter-story deformation of the superstructure changed slightly. The horizontal acceleration responses of 3DIB model decreased more than 60% and the vertical acceleration responses decreased more than 50% under the severe earthquake of 0.4g in PGA input, which confirmed that 3DIB could isolate both the horizontal and vertical earthquakes obviously.