This paper expounded the basic principle of the base-isolated structures. Established the equations of motion of single particle and multi-particle base isolation structural system. Adopted multi-layer and high-level framework groups, added laminated rubber bearings in the bottom respectively, input EL Centro seismic waves, and then the modal, time-history, base shear are compared and analyzed. By comparing the results obtained: base-isolated structure used in high-rise building can decrease a certain degree of the seismic response, in the multi-layer building the effect was more apparent. It also confirmed that the application of base isolation on multi-layer and high-level framework in the existing conditions still had some limitations.