Paper Title:
Microbial Community Analysis on Oxic-Settling-Anaerobic Process by Using PCR-DGGE Assay
  Abstract

In situ sludge reduction of the oxic-settling-anaerobic (OSA) process was investigated in this study and microbial community diversity in the system was analyzed by using a PCR-denaturing gradient gel electrophoresis (DGGE) approach. Comparing to the conventional activated sludge method, the production of excessive sludge in the OSA process was shown to be efficiently reduced by 44-50 % and the organic loading rate was observed to have a slight impact on sludge yield. As demonstrated by the slight variation of Shannon diversity indices (SDI), the dominant microbial composition remained stable in the OSA sludge with the increase of Ns. About 63% of clones represented by predominant bands in the DGGE pattern were affiliated with the subclass of b-proteobacteria. A number of bacteria in the OSA process were phylogenetically related to uncultured bacteria isolated from enhanced biological phosphorus removal (EBPR) sludge.

  Info
Periodical
Advanced Materials Research (Volumes 255-260)
Edited by
Jingying Zhao
Pages
2934-2939
DOI
10.4028/www.scientific.net/AMR.255-260.2934
Citation
J. F. Wang, Q. L. Zhao, "Microbial Community Analysis on Oxic-Settling-Anaerobic Process by Using PCR-DGGE Assay", Advanced Materials Research, Vols. 255-260, pp. 2934-2939, 2011
Online since
May 2011
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Price
$32.00
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