Fatigue performance of asphalt layer was studied by ALF full scale accelerated loading test. Fatigue performance characteristic of ALF test road was analyzed during accelerated loading. The relationship of road performance index of test period and accelerated loading times was established. Asphalt mixture laboratory four-point flexure beam fatigue test was processed by controlled strain mode. The relationship of field ALF test sections and laboratory fatigue test were built; the fatigue life of asphalt layer predicted methods were proposed.