Fatigue behaviors of prestressed concrete beams with different corrosion level are investigated in a series of bonded post-tensioned T-beams. The beams have the same designs and submitted to the same maximum and minimum load during the test. Accelerated corrosion method is used to make various corrosion rates of prestressed steel strands. With the corrosion level as main parameter, such as failure mode, fatigue life, mid-span deflection development, stiffness loss, stress expansion, are studied. The test results show that failure mode is changed fracture of non-prestressed steel bar into prestressed steel strand breaking with the increase of corrosion level. Corrosion reduces fatigue life and stiffness of beams, increase deflection and stress range of non-prestressed steel bar.