The dynamic globularization behavior during hot working of Ti-6Al-4V alloy was investigated by high temperature torsion tests. The torsion tests were carried out to investigate microstructure evolution occurring during dynamic globularization in Ti-6Al-4V alloy. The torsion tests were performed under a wide range of temperatures and strain rates with true strain up to 2. The flow curves revealed that the amount of flow softening for the fine alpha-lamellae structure was higher than that for the coarse alpha-lamellae structure under the temperature of 900oC. The effects of hot deformation parameters and initial microstructures on the dynamic globularization were analyzed.