The possibility for preparation of biodegradable film from cellulose-enrich waste was studied. This work dealt with the synthesis of cellulose film in LiCl/DMAc solvent system by microwave-induced esterification of cellulose from waste cotton fabric using DMAP and lauroyl chloride as a catalyst and esterifying agent, respectively. The cellulose laurate film was prepared by casting method. The structure of cellulose laurate was confirmed by FTIR and 1H-NMR analyses. The thermal stability was characterized by TGA. The optimum condition for esterification in terms of microwave power and time was investigated. The films obtained were tested for their tensile properties and potentiality of biodegradation.