Many concrete structures can not reach their designed service life because of their poor durability. Water and aggressive substances such as chloride ions are prerequisite to the deterioration of concrete. In aggressive environments, water repellent treatment is an effective path to delay the repair and improve the durability of concrete structures. Thousands of years ago, people used oils, animal fats or wax to treat the surface of natural stones, bricks or put some animal blood in mortar to make them water repellent. Many historical monuments were protected in these old methods. In this paper, different amounts of ox blood had been added in concrete. Compressive strength, water absorption and chloride penetration tests had been conducted. In this way, the influence of ox blood on water absorption of and chloride penetration into concrete had been investigated. The results indicated that the compressive strength of concrete with addition of ox blood decreased. And water absorption and chloride penetration into concrete were effectively reduced by adding some amounts of ox blood. The concrete with the surface abraded off 5mm, 15mm and 25mm which were mixed with ox blood had higher water absorption than concrete without abrasion.