Five beams were tested up to failure to study the shear behavior of concrete beams prestressed with fiber reinforced polymer (FRP). Different factors were taken into consideration: the type of prestressing tendons and the shear span ratio. The shear failure modes and the influence of different factors on shear behavior were investigated in details. The test results showed that FRP prestressed beams without stirrups had two shear failure modes: diagonal compression failure and shear compression failure; the shear span ratio was the most important factor to determine the failure mode and shear capacity of the prestressed beams. The shear capacity of concrete members prestressed with FRP tendons was lower than that of concrete beams prestressed with steel cables.