Human induced pluripotent stem cells is promising for regenerative medicine and tissue engineering. In this chapter, we focus on the culture and characteristics of human induced pluripotent stem cells. The induced pluripotent stem cells were plated on murine embryonic fibroblast feeder cells and expanded in human embryonic stem cells media contained basic fibroblast growth factor. The cells were passaged by collagenase IV digestion method and observed under invert microscope. The expression of alkaline phosphatase was detected by immunocytochemistry. The cultured induced pluripotent stem cells grew well and stability with similar characteristics of human embryonic stem cells. These cells also expressed alkaline phosphatase. They formed embryoid body in feeder-free and suspension culture conditions. The results provide an experimental basis for improvement of induction study and further application to generate patient-specific induced pluripotent stem cells.