A kind of cooperative lesson preparation mode for electronic experimental teaching was put forward in this paper aimed at solving some common issues in electronic experimental teaching. For example, electronic experimental teaching has lagged behind the development of electronic technology, teachers have been exposed to great pressure and therefore perform poor lesson preparation, teaching and research levels of full-time experimental teachers have been unsuitable for new situations, etc. Correspondingly, a cooperative lesson preparation mode of electronic experimental teaching was proposed. The mode involved a series of steps and forms such as clarifying objectives, exerting individuality, sharing resources, authenticating each other, evaluating and reflecting, etc. Teachers were inspired and exerted in passion and potential fully by grasping and using some rules and functions in psychology, efficiency and non-cognitive characters. During practice of two semesters, teaching and research atmosphere of the circuit center has become increasingly thick and teaching quality has improved significantly.