Paper Title:
Development of a RT-PCR for the Detection of European Brown Hare Syndrome Virus
  Abstract

To develop the reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of European brown hare syndrome virus (EBHSV), 3 specific primers and 9 overlapping oligo primers were designed to amplify a fragment of 335 nucleotides from the genome of EBHSV according to the genome sequences published in GenBank, and a 359 bp DNA fragment of the capsid protein(VP60)gene of EBHSV was synthesized in vitro by using overlap extension PCR to construct the recombinant plasmids pGM-T-EBHSV, then the RT-PCR method was described through the optimization of reaction conditions, sensitivity and specificity tests. Results showed the RT-PCR method was supplied for the detection of EBHSV with good specificity and sensitivity. The sensitivity of the RT-PCR could reach about 50 copies of cloned viral genomic fragments (pGM-T-EBHSV), and no amplifications for RHDV, Pasteurella multocida, E.coli and Salmonella from rabbits detection by this approach.

  Info
Periodical
Advanced Materials Research (Volumes 271-273)
Edited by
Junqiao Xiong
Pages
410-416
DOI
10.4028/www.scientific.net/AMR.271-273.410
Citation
Y. Z. Xiao, L. R. Li, Y. Wang, X. P. Yao, X. Q. Han, Z. Q. Yang, B. Zhou, K. Y. Wang, Y. M. Zhang, "Development of a RT-PCR for the Detection of European Brown Hare Syndrome Virus", Advanced Materials Research, Vols. 271-273, pp. 410-416, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Ai Li, Guo Xing Yang, Wei Zhang
Abstract:To establish a rapid, sensitive and specific multiplex PCR method for the simultaneous detection of Staphylococcus aureus,...
2803
Authors: Ji Ming Yan, Xiao Hong Shi, Miao Mei, Hong Bo Dai, Hua Zhi Ye
Abstract:Plasmodiophora fire of broad bean is responsible for Olpidium Viciae Kusano, which is a kind of Fungi subdivided into bacteria flagellum...
507
Authors: Wan Li, Xiu Hong Xu, Jia Lei Xiao, Bi Xian Zhang, Hong Tao Li, Yong Cai Lai
Chapter 7: Pollution Control Project (Air, Water, Solid)
Abstract:Like bacteria, fungi play an important role in the composting process as major decomposers of organic substances. As only a small fraction of...
1747
Authors: Xiao Hua Yang, Xiao Feng Wei, Jian Hua Hu, Xiong Wei Liu, Shao Jin Chen
Chapter 2: Biology Technology and Materials
Abstract:Swine and canine influenza, which is acute viral respiratory infectious disease, can do great threat to the health of people and animals....
733
Authors: Yu Fen Jin, Yan Lei Li, Yan Hua, Xiao Gang Zhang, Ting Yu
Chapter 6: Biological Chemistry, Pharmaceutical, Medicine and Biomedical Engineering
Abstract:Objective To evaluate the effectiveness of prokaryotic expression of fusion proteins pp150-pp65 of human cytomegalovirus (hCMV) for its...
1313