Paper Title:
Amplifying and Sequencing Analysis the Internal Transcribed Spacer (ITS) Regions of Olpidium Viciae Kusano’s Ribosomal DNA in Broad Bean
  Abstract

Plasmodiophora fire of broad bean is responsible for Olpidium Viciae Kusano, which is a kind of Fungi subdivided into bacteria flagellum amon. We have developed a polymerase chain reaction based method for the rapid identification internal transcribed spacer (ITS) regions of productionally significant fungi Olpidium Viciae Kusano from areas of 2500~3000 metres above sea level. Sequences of the nuclear internal transcribed spacer (ITS) regions ITS1 and ITS4 have been used widely in molecular characteristic studies because of their relatively high variability and facility of amplification. A universal quickly SDS micro-DNA extraction method was used combining a RNaseA pretreatment step to remove PCR interferential RNA. Target sequences in ITS regions genomic were amplified by PCR and sequenced. Using Hanpanchun lesion and healthy bean leaves as template and ITS1, ITS4 as primer to amplify ITS region, the results revealed ITS gene of broad bean genome could be amplified with size of 750bp from healthy leaves, it could be amplified two fragments of 750bp and 500bp from the DNA template extracted from Hanpanchun lesion tissue. The ITS sequence of Olpidium Viciae Kusano is 99% homoeology with Cercospora (grey speck) pathogen. This may lay the foundation for research about classification and analyze evolutionary relationships of Olpidium Viciae Kusano.

  Info
Periodical
Advanced Materials Research (Volumes 271-273)
Edited by
Junqiao Xiong
Pages
507-513
DOI
10.4028/www.scientific.net/AMR.271-273.507
Citation
J. M. Yan, X. H. Shi, M. Mei, H. B. Dai, H. Z. Ye, "Amplifying and Sequencing Analysis the Internal Transcribed Spacer (ITS) Regions of Olpidium Viciae Kusano’s Ribosomal DNA in Broad Bean", Advanced Materials Research, Vols. 271-273, pp. 507-513, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yong Feng Li, Yi Xuan Wang, Lu Wang, Zhan Qing Wang
Environmental Biotechnology
Abstract:To develop the identification of species for fermentative biohydrogen-producing bacterium, scholars have found a method which is based on PCR...
1413
Authors: Ai Li, Guo Xing Yang, Wei Zhang
Abstract:To establish a rapid, sensitive and specific multiplex PCR method for the simultaneous detection of Staphylococcus aureus,...
2803
Authors: Wan Li, Xiu Hong Xu, Jia Lei Xiao, Bi Xian Zhang, Hong Tao Li, Yong Cai Lai
Chapter 7: Pollution Control Project (Air, Water, Solid)
Abstract:Like bacteria, fungi play an important role in the composting process as major decomposers of organic substances. As only a small fraction of...
1747