Based on a series of single factor experiments, response surface methodology was employed to predict optimum conditions for domestication of lactic acid bacteria to prepare jujube lactic acid fermented beverage. A central composite design involving jujube puree content, fermentation temperature and inoculum size was used, and second-order model for viable biomass was employed to generate the response surface. The optimized conditions were as follows: jujube puree content 71.85 %, fermentation temperature 41.57°C and inoculum size 3.66%. The predicted viable biomass under the conditions was 3.099×108 cfu/mL. The practical conditions were modified as follows: jujube puree content 72 %, fermentation temperature 41°C, and inoculum size 3.7% (fermentation time 8 h).Experimental verification gave the value of 2.99×108 cfu/mL.