Reclaimed water contains a mass of pathogenic microorgansims, one of the main concerns for reclaimed water reuse is the microbiological water quality and thereby the possibility of spreading diseases. In recent years, most researches focused on deep treatment of waste water. However, few studies have considered variation mechanism and transport of pathogenic microorgansims in different environment .With the more and more application of reclaimed water in mining field, we should pay more attention to hygienic safety. The inactivation by temperature, grinding, flotation reagents and adsorption of pathogens with mineral particulates are of particular importance in determining pathogens transport and distribution. Consequently, based on a synthetical pathogenic microorgansims index, study should center on the effect of floatation environment and floatation process on survive of pathogenic microorgansims and variations mechanism; the fate, temporal and spatial distribution of pathogenic microorgansims in floatation system; transport disciplinarian of pathogen in floatation process. The result of this study provides a basis and technical advice for reclaimed water safety renovation and wastewater treatment technology.