Paper Title:
Recognition of Papain by Konjac Glucomannan-Based Molecularly Imprinted Membrane
  Abstract

Objective: To prepare a novel protein molecularly imprinted membrane. Methods: Konjac glucomanan was swollen, cross-linked, added papain and glycerol, dried to form imprinted membranes. Results: After eluted, the rebinding adsorption amounts of papain for imprinted and blank membranes were (12.692±3.452) μg/cm2 and (7.849±2.321) μg/cm2 respectively. The papain imprinted membrane had the highest imprinted factor α 1.8 for papain than for other proteins. Results of FT-IR and SEM showed that the cavity structure of the eluted membrane provided space for rebinding. Conclusions: Konjac glucomannan-based imprinted membrane has good specific adsorption and selective recognition abilities for the templates- papain, and shows great possibilities to be a promising protein molecularly imprinted polymer.

  Info
Periodical
Advanced Materials Research (Volumes 282-283)
Chapter
Chapter 4: Intelligent Materials, Photonics and Power
Edited by
Helen Zhang and David Jin
Pages
687-690
DOI
10.4028/www.scientific.net/AMR.282-283.687
Citation
Z. Y. Mi, Z. Ma, X. L. Li, W. D. Xiong, Y. Q. Zhang, "Recognition of Papain by Konjac Glucomannan-Based Molecularly Imprinted Membrane", Advanced Materials Research, Vols. 282-283, pp. 687-690, 2011
Online since
July 2011
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Price
$32.00
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