Paper Title:
Directing Human Chondrocytes Cell Behavior by Fabrication of Protein Micropattern on Polystyrene Surfaces
  Abstract

In the present study, three BMP-2 (bone morphogenetic protein-2) micropatterns were produced on polystyrene (PS) surface by microcontact printing in order to investigate the effect of protein micropattern on biomaterials surface on human chondrocytes behavior. The excellent BMP-2 micropatterns from fluorescent observation were successfully obtained. Cell adhesion and immunohistochemical assay were utilized to explore cell behavior. The results indicated that the protein micropatterns have significant influence on cell adhesion, spread, alignment and protein expression. The cells preferentially adhered on protein micropattern zones. The cells tend to spread towards micropattern direction, leading to better spread. The spread cells could excreted more type II and VI collagen than that of non-spread or poor-spread cells. The results of this paper suggested that extracellular matrix (ECM) protein micropatterns can effectively regulate human chondrocytes behavior, which may be helpful for development of new cartilage tissue.

  Info
Periodical
Advanced Materials Research (Volumes 284-286)
Chapter
Biomaterials
Edited by
Xiaoming Sang, Pengcheng Wang, Liqun Ai, Yungang Li and Jinglong Bu
Pages
1815-1818
DOI
10.4028/www.scientific.net/AMR.284-286.1815
Citation
C. J. Pan, Y. D. Nie, Y. X. Dong, "Directing Human Chondrocytes Cell Behavior by Fabrication of Protein Micropattern on Polystyrene Surfaces", Advanced Materials Research, Vols. 284-286, pp. 1815-1818, 2011
Online since
July 2011
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Price
$32.00
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