PA66 nanocomposites with various contents of TiO2 were prepared by twin-screw extruder in order to study the influence of inorganic nanoparticles on tribological properties of PA66melt-intercalation method. The effects of content of nanoparticles and load on friction coefficient and wear volume loss were investigated, respectively. The tensile properties of PA66/TiO2 nanocomposites were greatly improved. The tribological behaviors of the PA66/TiO2 nanocomposites against steel ring were evaluated on a block-on-ring type (M-2000) wear tester. Nano TiO2 at 1vol.% was effective for improving the tribological properties of neat PA66, because the nano TiO2 at the low content may act as the reinforcing element to bore load and thus decrease the plastic deformation.