Apparel textiles in service will adsorb metabolism perspiration from human body, thus prompting microbial propagation and leading to fiber degradation and splash. More seriously, this microbe gives rise to allergy and red swelling on human skin, which makes our body unwell. Staphylococcus aureus is one of common bacteria on human skin, but its excessive breeding on skin will bring red swelling and inflammation. In order to avoid this situation happening, we add antibacterial ingredients in textiles which were not harmful to human and cannot have negative effect on human. And this study aims at seeking for anti-staphylococcus aureus plants, trying to add ingredients from plants in textiles to discuss their antimicrobial change and investigating whether to be potential in textiles application. Phyllanthus Urinaria Linn (PUL) is common in tropical East Asia and can suppress the growth of bacteria regarding staphylococcus aureus, typhoid bacillus, pseudomonas aeruginosa etc. It is shown that when PUL was treated at 60°C for 6 hours in 300 ml 95 % ethyl alcohols, extract rate was up to 12.53 %. And qualitative antimicrobial was effective between 12g/100ml and 0.09375g/100ml PUL extract liquor. And the fabric qualitative antimicrobial was obviously valid when extract concentration was above 0.75g/100 ml. Therefore, cotton treated by 1.5g/100ml PUL extract liquor had prominent antimicrobial effect.