Acid-activated by H2SO4 was applied to modify bentonite. Acid-activated bentonite supported Au catalyst was prepared by deposition-precipitation and compared with SiO2 supported one. CTAB was used to modify the surface of acid-activated bentonite. Au-Ce/bentontie catalyst was prepared by adding Ce into catalyst as assistant. CO oxidation was used to evaluate the catalytic activity of samples. These samples were characterized by BET, XRD, ICP and CO-TPD. The activity results showed that Au/Bentonite was more active than Au/SiO2. The BET results showed that the surface area and pore volume of acid-activated bentontie had a substantial improvement. Using the bentontie acid-activated by 30wt%H2SO4 as supporter，the Au-Ce catalyst has a better catalytic performance than Au catalyst.