Paper Title:
Enhanced the Insulin Secretion of HIT-T15 Cells by Hyaluronic Acid-Coating Involves Cytosolic Free [Ca2+]i Response
  Abstract

Hyaluronic acid (HA) is an extremely ubiquitous polysaccharide of extracellular matrix in body and plays various biological roles. In this study, cell viability, insulin secretion and cytosolic free Ca2+ concentration were investigated in HIT-T15 cells after HA-treatment. The cell viability was determined by the MTT assay while the insulin secretion was detected by ELISA technique. The Flou-3/AM was applied to probe intracellular free calcium concentration. HA (0.25, 0.5, 1.0 mg/dish) increase insulin secretion in the 3 or 25 mM glucose-stimulation for 4 h. Cytosolic Ca2+ fluorescent intensity in HA-treatment groups (0.25, 0.5, 1.0 mg/dish) is significantly higher than that in control group. This study implied that the increasing of insulin secretion of HIT-T15 cells by HA-coating result from the enhancement of cytosolic Ca2+. And these data also provide some information on design biocompatibility when HA used as a biomaterial for bioartificial pancreas.

  Info
Periodical
Advanced Materials Research (Volumes 287-290)
Chapter
Biomaterials
Edited by
Jinglong Bu, Pengcheng Wang, Liqun Ai, Xiaoming Sang, Yungang Li
Pages
1960-1965
DOI
10.4028/www.scientific.net/AMR.287-290.1960
Citation
Y. P. Li, B. Gu, X. F. Pi, Y. C. Gong, X. Y. Xiong, G. J. Wu, Z. L. Li, "Enhanced the Insulin Secretion of HIT-T15 Cells by Hyaluronic Acid-Coating Involves Cytosolic Free [Ca2+]i Response", Advanced Materials Research, Vols. 287-290, pp. 1960-1965, 2011
Online since
July 2011
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Price
$35.00
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