Un-promoted and promoted Co/SiO2 catalysts with ZrO2 and Ru were prepared and the catalytic performances for Fischer-Tropsch synthesis were investigated. The prepared catalysts were characterized by XRD, N2 adsorption/desorption, and X-ray absorption techniques. XRD suggested that the cobalt species presence in all prepared catalysts was Co3O4. Promoted catalyst with ZrO2 and Ru showed high distribution of cobalt on silica surface resulted in smaller Co3O4 crystalline size than un-promoted ones. From the reaction testing, the obtained products of C1 to C9 from un-promoted and promoted Co/SiO2 were studied.