To prepare molecularly imprinted polymers (MIPs) with good recognition properties，the effect on the MIPs adsorption performance were investigated from the different functional monomers. The MIPs were synthesized by using andrographolide as template, and α-methyl acrylic acid (MAA), acrylamide (AM) and 1 - vinylimidazole were used as functional monomer. Before polymerization, the interaction between the template molecule and the two functional monomers was studied by UV spectrum. After polymerization the different adsorption properties of MIPs prepared by the three types of functional monomers were compared by equilibrium binding. The results showed that the adsorption properties of the MIPs by 1-vinyl imidazole as functional monomer were similar to the MIPs by AM as functional, which the former is slightly higher than the latter and significantly higher than that of the one by MAA as functional monomer.