Paper Title:
Electrochemical Inspection of the Interaction of Double Strand ds-DNA with Carmine on Nanometer TiO2 Doped Carbon Paste Electrode
  Abstract

The interaction of the single azo dye, carmine with fish-sperm dsDNA is inspected in pH 3.2 H2SO4 with electrochemical method on the surface of nanometer TiO2 modified carbon paste electrode. After the addition of dsDNA, the peak currents of oxidation and reduction peaks of carmine decrease with a positive shift of potential, indicating that intercalation interaction between the dye and dsDNA is taken place. This is consistent with fluorescence spectra results. The binding constant and binding ratio is calculated as 4.92×108 and 1:2, respectively. Furthermore, the decrease in the oxidation peak currents is found proportional to dsDNA concentration in the range of 21.24-127.44 μg·mL-1 with a detection limit of 16.04 μg•mL-1.

  Info
Periodical
Advanced Materials Research (Volumes 287-290)
Chapter
New Functional Materials
Edited by
Jinglong Bu, Pengcheng Wang, Liqun Ai, Xiaoming Sang, Yungang Li
Pages
37-42
DOI
10.4028/www.scientific.net/AMR.287-290.37
Citation
M. M. Ma, Z. Tong, J. B. Yang, "Electrochemical Inspection of the Interaction of Double Strand ds-DNA with Carmine on Nanometer TiO2 Doped Carbon Paste Electrode", Advanced Materials Research, Vols. 287-290, pp. 37-42, 2011
Online since
July 2011
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Price
$32.00
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