Vibration testing and modal experiment under the different speed are carried out to the high speed motorized spindle whose maximum speed is 60 000 rpm. Time domain waveform and spectrum of motorized spindle’s radial vibration acceleration are obtained under the unload state. Through calculating and analyzing, the vibration velocity and the testing point which generates the maximum vibration velocity are found out. Then motorized spindle is validated whether it accords with the requirements of high-precision manufacture. And the correctness of the vibration testing method is also verified. Primary reasons of the motorized spindle’s vibration are put forward. The first five steps natural frequencies of motorized spindle test-bed are gained by appropriate ways to checkout if the test-bed in the resonance region.