Clothing combining knitted and woven fabrics gets more and more favor of people. In this study, three knitted fabrics and three woven fabrics which are common on market were selected and seamed. The tensile breaking strength, elongation at break and the fracture reason of the joint of knitted and woven fabrics were tested using the one-way vertically stretching method. The test results were analyzed and it was found there are many factors affecting the seam strength of knitted and woven fabrics. Experiment shows that the main factors include: stitch density, joint method and choice of the joining material. The study of this subject provides the basis for designing better joint wear and improving sewing quality and efficiency.