The microstructure and the formation mechanism of martensite in W6Mo5Cr4V2 steel was studied by metallographic microscope and JEM-2100 transmission electron microscope after the samples were austenized between the temperatures of Ac1~Accm and then quenched. The results show that When heating W6Mo5Cr4V2 steel samples between the temperatures of Ac1~Accm and then quenching, the cryptocrystalline martensite will be obtained. The cryptocrystalline martensite is plate martensite actually. It is considered that the formation cause of the cryptocrystal martensite is extremely inhomogeneous chemical composition in the austenite grains and the difference of martensite starting point (Ms point) of every small area in austenite grains. Besides the high-density dislocation and the fine twin crystal, the substructure of the cryptocrystalline martensite includes the superfine stacking fault. The stacking fault is caused by the stacking misarrangement during the crystal lattice reconstruction of martensite phase transformation. The midrib exists in the cryptocrystal martensite of W6Mo5Cr4V2 steel, which is composed of the fine twin crystal plates. The shear mechanism can not account for the formation of the martensite midrib.