The tension-torsion thin walled tube specimens were used as the researching object in this paper. The method of determination to the critical plane which has the maximum normal strain and maximum shear strain was expounded. The strain state on the critical plane under non-proportional loading was analyzed, and the unified prediction model was used to calculate the fatigue life. In order to research the influence of phase difference on fatigue life under the non-proportional loading, the relation of the equivalent strain and the phase difference in different positive strain amplitude and different strain amplitude ratio were analyzed. It’s found that the dangerous phase difference which has the shortest fatigue life is in direct relation with the strain amplitude ratio. The general formula of dangerous phase difference is presented. Through the material mechanics performance and fatigue parameters of uniaxial stress state, the coefficients in the formula can be obtained and the coefficients of 15 kinds of common materials are given for practical application.