Paper Title:
DNA Electrochemical Biosensor Based on Chitosan and Gold Nanoparticles Using Methylene Blue as Electrochemical Indicator
  Abstract

In this paper, a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology was reported. Gold nanoparticles were firstly immobilized on chitosan modified gold electrode by large amino of chitosan because of strong electrostatic adsorption effect between amino and gold atom, and thoil group modified DNA (SH-ssDNA) probe sequence was then self-assembled onto the electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied to investigate the electrochemical behavior of different modified electrodes with methylene blue as hybrid indicator. The results in optimization experiment condition show that: The peak current difference value pre and post hybridization was linearly related to the logatithmic value of the target DNA concentration ranging from 10-8~10-5mol/L.A detection limit of 3.55×10-9mol/L can be estimated.

  Info
Periodical
Edited by
Tianbiao Zhang and Jerry Tian
Pages
128-134
DOI
10.4028/www.scientific.net/AMR.298.128
Citation
A. C. Zhang, C. Zhou, "DNA Electrochemical Biosensor Based on Chitosan and Gold Nanoparticles Using Methylene Blue as Electrochemical Indicator", Advanced Materials Research, Vol. 298, pp. 128-134, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Mei Ma, Xiao Ping Fan, Zhao Dai, Xiao Qing Wang, Qing Yin Zhang
Abstract:A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) functionalized with a mercapto group...
56
Authors: Yan Liu, Zhao Dai, Shi Chao Xu, Ji Mei Zhang, Guo Zheng, Qing Yin Zhang, Guang Ping Chen
Abstract:A novel electrochemical DNA biosensor system based on Au nanoparticles (AuNPs) modified Au electrode and anthraquinone-2,6-disulfonic acid...
78
Authors: Cun Zhou, Ai Chun Zhang
Abstract:In this paper, an efficient process for preparing monodisperse gold nanoparticles coated with silica shells (Au@SiO2) was reported, and a...
135
Authors: Yong Zhou
Chapter 2: Smart Materials for Biomedical Engineering Application
Abstract:A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and...
331