Paper Title:
DNA Electrochemical Biosensor Based on Chitosan and Core-Shell Au@SiO2 Nanoparticles
  Abstract

In this paper, an efficient process for preparing monodisperse gold nanoparticles coated with silica shells (Au@SiO2) was reported, and a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology as well as Au@SiO2 nanoparticles was presented. Chitosan was immobilized on gold electrode by good film-forming property and lots of amino because of strong electrostatic adsorption effect between amino and gold atom, Au@SiO2 nanoparticles were also immobilized to the electrode in that it kept the excellent features of gold nanoparticles, therefore, thoil group modified DNA (SH-ssDNA) probe sequence can self-assembled on Au@SiO2 nanoparticles modified electrode with the purpose of realize quantitative detection of complementary DNA. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied to investigate the electrochemical behavior of different modified electrodes with methylene blue as hybrid indicator. The results in optimization experiment condition show that: the peak current difference value pre and post hybridization was linearly related to the logatithmic value of the target DNA concentration ranging from 10-10~10-6mol/L. A detection limit of 4.02×10-11mol/L can be estimated.

  Info
Periodical
Edited by
Tianbiao Zhang and Jerry Tian
Pages
135-141
DOI
10.4028/www.scientific.net/AMR.298.135
Citation
C. Zhou, A. C. Zhang, "DNA Electrochemical Biosensor Based on Chitosan and Core-Shell Au@SiO2 Nanoparticles", Advanced Materials Research, Vol. 298, pp. 135-141, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Chun Xiu Liu, Hong Min Liu, Qing De Yang, Nan Sen Lin, Yi Lin Song, Li Wang, Xin Xia Cai
Abstract:A biosensor based on gold electrode modified by Pt nanaoparticles/Osmium redox polymer/Nafion trilayer film was fabricated and used for...
311
Authors: Mei Ma, Xiao Ping Fan, Zhao Dai, Xiao Qing Wang, Qing Yin Zhang
Abstract:A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) functionalized with a mercapto group...
56
Authors: Yan Liu, Zhao Dai, Shi Chao Xu, Ji Mei Zhang, Guo Zheng, Qing Yin Zhang, Guang Ping Chen
Abstract:A novel electrochemical DNA biosensor system based on Au nanoparticles (AuNPs) modified Au electrode and anthraquinone-2,6-disulfonic acid...
78
Authors: Ai Chun Zhang, Cun Zhou
Abstract:In this paper, a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology was reported. Gold nanoparticles were...
128
Authors: Yong Zhou
Chapter 2: Smart Materials for Biomedical Engineering Application
Abstract:A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and...
331