Paper Title:
Novel DNA Electrochemical Biosensor Using Anthraquinone-2-Sulfonic Acid Sodium Salt as Hybridization Indicator
  Abstract

A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) functionalized with a mercapto group (SH-MWNTs) and gold nano-particles (GNPs) was presented, where anthraquinone-2-sulfonic acid sodium salt (AQMS) was used as hybridization indicator. The differential pulse voltammetry responses demonstrated that this DNA/GNPs/SH-MWCNTs/Au biosensor was enabled to specifically detect the single-base mismatch DNA sequence in phosphate buffer solution with pH 7.4 containing 0.3 mol/L Na+ and 1.0 mmol/L AQMS. The result showed that when the target DNA concentration was 1.0×10-10 to 1.6×10-5 mol/L, the cathodic peak current of Au electrode system with AQMS as indicator was linearly related to complementary NDA concentration, and the detection limit was about 3.82×10-11 mol/L and had good stability and specificity.

  Info
Periodical
Edited by
Tianbiao Zhang and Jerry Tian
Pages
56-62
DOI
10.4028/www.scientific.net/AMR.298.56
Citation
M. Ma, X. P. Fan, Z. Dai, X. Q. Wang, Q. Y. Zhang, "Novel DNA Electrochemical Biosensor Using Anthraquinone-2-Sulfonic Acid Sodium Salt as Hybridization Indicator", Advanced Materials Research, Vol. 298, pp. 56-62, 2011
Online since
July 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Chun Xiu Liu, Hong Min Liu, Qing De Yang, Nan Sen Lin, Yi Lin Song, Li Wang, Xin Xia Cai
Abstract:A biosensor based on gold electrode modified by Pt nanaoparticles/Osmium redox polymer/Nafion trilayer film was fabricated and used for...
311
Authors: Yan Liu, Zhao Dai, Shi Chao Xu, Ji Mei Zhang, Guo Zheng, Qing Yin Zhang, Guang Ping Chen
Abstract:A novel electrochemical DNA biosensor system based on Au nanoparticles (AuNPs) modified Au electrode and anthraquinone-2,6-disulfonic acid...
78
Authors: Ai Chun Zhang, Cun Zhou
Abstract:In this paper, a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology was reported. Gold nanoparticles were...
128
Authors: Cun Zhou, Ai Chun Zhang
Abstract:In this paper, an efficient process for preparing monodisperse gold nanoparticles coated with silica shells (Au@SiO2) was reported, and a...
135
Authors: Yong Zhou
Chapter 2: Smart Materials for Biomedical Engineering Application
Abstract:A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and...
331