Hemocompatibility is a key property of biomaterials that come in contact with blood. Surface modification has shown great potential for improving the hemocompatibility of biomedical materials and devices. It has been improved that TiO2 has excellent hemocompatibility. In this study, TiO2 nanoparticles was added into polytetrafluorethylen (PTFE) resin in order to enhance hemocompatibility of the organic coating. The surface hydrophobicity of the coatings was characterized by contact angle measurement. Systematic evaluation of hemocompatibility, including platelet adhesion and blood clotting, proved that TiO2 nanoparticles composite PTFE coating have better hemocompatibility. It is suggested that TiO2 nano particles can improve hemocompatibility of organic coating due to its passive surface and chemical stability.