For its advantages of simple structure and low cost, turbine flow sensor has been widely applied in gas and liquid transportation and other industries for measuring liquid or gas flowrate. Firstly, the output pulse signal property of turbine flow sensor was studied. To achieve the dynamic response, a new turbine flow sensor which based on the photoelectric detection is designed in this paper. In addition, experimental testing on the turbine flow sensor has been carried out on standard calibration facilities with high accuracy. The results confirm that the design is feasible, and the repeatability error is about 0.5%, the maximum relative error is less than 1%.