Paper Title:
Electrochemical DNA Biosensor for the Detection of Short DNA Species by Using Compound Co(phen)32+
  Abstract

The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridization events in a more rapid, simplistic and cost-effective manner compared to conventional methods. Here, we present an electrochemical DNA biosensor for the specific and quantitative detection of single-stranded DNA (ssDNA). Probe oligonucleotides were immobilized onto gold electrodes by a 5′ end thiol-group linker. Following hybridization with the complementary DNA, the cobalt complex was electrochemically accumulated on the double-stranded DNA layer and the differential pulse voltammogram (DPV) for this electrode gave an electrochemical signal due to the redox reaction of [Co(phen)3]2+/3+ that was bound to the double-stranded DNA on the electrode, and it can sense single base-pair mismatche within the sequence of the hybridized double-stranded DNA (dsDNA).

  Info
Periodical
Advanced Materials Research (Volumes 301-303)
Chapter
Chapter 1: Material Science and Technology
Edited by
Riza Esa and Yanwen Wu
Pages
213-218
DOI
10.4028/www.scientific.net/AMR.301-303.213
Citation
C. Zhou, B. W. Cheng, X. P. Fan, Z. Dai, G. Zheng, Q. Y. Zhang, G. P. Chen, "Electrochemical DNA Biosensor for the Detection of Short DNA Species by Using Compound Co(phen)32+", Advanced Materials Research, Vols. 301-303, pp. 213-218, 2011
Online since
July 2011
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Price
$32.00
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