Paper Title:
Magneticbead-Based Enzyme-Linked Immunoassay for Detection Carcinoembryonic Antigen in Gastric Cancer Cells
  Abstract

A sensitive immunoassay based on immunomagnetic beads for measuring carcinoembryonic antigen (CEA) concentration in cancer cells was presented in this article. We used immunomagnetic beads to separate CEA in the gastric cancer cells and those CEA could be marked with horseradish peroxidase (HRP) labeled anti-CEA secondary antibody. The HRP could catalyze non-fluorescent 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) with hydrogen peroxide (H2O2) to fluorescent products. Therefore, the CEA concenctration was quanlified by flourescence intensity at 585 nm emission wavelength. The flourescence intensity of the product responged well linearly to CEA concentration in the range from 3.0 to 60 ng/mL with a lower detection limit of 1.5 ng/mL(3σ). This method could be applied to determine tumor markers in gastric cancer cells.

  Info
Periodical
Advanced Materials Research (Volumes 306-307)
Chapter
Chapter 4: Superconductive and Magnetic Materials
Edited by
Shiquan Liu and Min Zuo
Pages
393-397
DOI
10.4028/www.scientific.net/AMR.306-307.393
Citation
F. Q. Xia, Y. N. Yuan, "Magneticbead-Based Enzyme-Linked Immunoassay for Detection Carcinoembryonic Antigen in Gastric Cancer Cells", Advanced Materials Research, Vols. 306-307, pp. 393-397, 2011
Online since
August 2011
Export
Price
$32.00
Share

In order to see related information, you need to Login.

In order to see related information, you need to Login.

Authors: Yu Xi Yu, Xian Qiao Liu
Abstract:A rapid and simple antibody purification method is reported for the isolation of monoclonal antibody IgG1 from SARS-CoV N protein rabbit...
2146
Authors: Ji Lei Wang, Ming Hui Li, Li Gang Wei, Ying Chong Ma, Kun Lan Li, Ya Chen Yu
Chapter 13: Structural Materials and Biomaterials
Abstract:Biodegradable cellulose/collagen beads were successfully prepared by coagulating a blend of cellulose and collagen using...
2365
Authors: Shu Xian Chen, Jing Liu, Dao Dong Pan, Ning Gan
Chapter 1: Sensors and Materials for Sensors, their Applications
Abstract:An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal...
56