Bovine Serum Albumin Adhesion Force Measurements Using an Atomic Force Microscopy |
| Journal |
Advanced Materials Research (Volume 32) |
| Volume |
Frontiers in Materials Science and Technology |
| Edited by |
John Bell, Cheng Yan, Lin Ye and Liangchi Zhang |
| Pages |
49-52 |
| DOI |
10.4028/www.scientific.net/AMR.32.49 |
| Online since |
February, 2008 |
| Authors |
Chun Chih Lai, John M. Bell, Nunzio Motta |
| Keywords |
Atomic Force Microscopy, Bovine Serum Albumin, Protein Adhesion |
| Abstract |
A new, direct method has been developed to measure the adhesion forces of bovine
serum albumin (BSA) on surfaces by using Atomic Force Microscopy (AFM) in liquid environment.
We were able to measure interactions between proteins and substrate surface in PBS solution
directly without any modification to the substrate or the AFM tip. Two different surfaces have been
used in the experiments: mica (hydrophilic surface) and polystyrene (hydrophobic surface). The
results show that a polystyrene surface is more adhesive to BSA than a mica surface. This is
consistent with previous research, which assessed that hydrophobic surfaces enhance protein
adhesion but hydrophilic surfaces do not, demonstrating the effectiveness of the technique. |
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