Objective: To evaluate the effects of amniotic extracellular matrix (AECM) on the repairation of facial nerve in rabbits. Methods: The transected nerve ends of the facial nerves of rabbits were then repaired with the AECM nerve conduit or an autologous nerve graft. After 3 months, the animals’ neural conductive velocity were determined. The myelinated fibers across the specimen were counted with histological examination. Results: The total count of medullated nerve fibers varied significantly, but the nerve conduction velocity had no significance between the AECM grafts and the autologous nerve grafts. Conclusion: AECM could become the biocompatible material for repairing the peripheral nerves.