The dyeing accelerating effect of octyl trimethyl ammonium bromide (OTAB) on dyeing silk with reactive red dye B-3BF, reactive yellow dye B-4RFN and reactive dark blue dye B-2GLN was studied in this paper. The dye-uptake rates increased with increasing concentration of OTAB, and the highest dye-uptake rates of three reactive dyes were 91~98%. The adsorption kinetics of dyeing process at different temperatures was also investigated by using pseudo first- and second-order kinetic models. The results showed that adsorption rates increased with increasing temperature. The adsorption kinetics of three reactive dyes on silk with OTAB as accelerant was found to follow the pseudo second-order sorption kinetics equation. The activation energy of reactive red dye B-3BF, reactive yellow dye B-4RFN and reactive dark blue dye B-2GLN dyeing on silk with OTAB as accelerant were found to be 26.91, 17.31, 6.99 kJ/mol, respectively.