The objective of this work was to use a novel 3-D test analysis system for evaluating the mechanical properties of the natural fiber fabric composites under biaxial loads. Composites with three resin matrices (water-based epoxy resin, isocyanate resin, phenolic resin) were investigated, and strain filed were characterized by using the digital speckle correlation. The water-based epoxy resin plate and isocyanate resin plate demonstrated a characteristic of orthotropy and elastoplasticity, while the phenolic resin board revealed linear elastic and brittle-fracture simultaneously at X,Y-axial. Dissimilarities of biaxial load value were related to the orthotropy of composite structure, and load changes in fracture direction had a negative effect on the other directions at breaking moment. The degrees of dropping presented a positive correlation with the load values at rift direction. Under the linear elastic stage, the value of load and average strain at Y-direction were larger than that under X-direction within the same testing area. The strain-filed at X/Y-direction provided by isocyanate and phenolic resin plates illustrated a more smooth change than that of the water-based epoxy resin plate.