This research studies the effect of penetrant, formic acid, sodium bicarbonate and steaming time on the rubbing fastness and K/S value of printed leather. The results show that the optimum concentration of sodium bicarbonate is 10 g/L in the first step of the leather pre-treatment. The optimum concentration of penetrant is 20g/L and the PH of pretreatment solution is 3 in the second step of the pre-treatment. The wet and dry rubbing fastness reaches grade 3 and 4-5. The pattern on the printed leather is clear and fine.