This work focused on the release behaviour and antibacterial efficiency of Polypropylene (PP)-based and Polyethylene terephthalate (PET)-based hollow fiber with silver particles incorporated (shortened as PP/Ag and PET/Ag). Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were used to investigate the antibacterial capability of Ag/PP and Ag/PET with the method of antibacterial kinetics experiments. The antibacterial results demonstrated that all samples had an excellent antibacterial property against E. coli and S. aureus, and the antibacterial efficiency of Ag/PET was greater than that of Ag/PP. Furthermore, Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AS) was applied to detect the release behaviour of silver ions from Ag/PP and Ag/PET. And the results showed that in the same weight of silver incorporated in hollow fibers, the release behaviour of silver ions from Ag/PET was more than that from Ag/PP , which may be explained by the moisture absorption of the PP and PET. From the results of antibacterial and release experiments, it could be concluded that the release behaviour of silver ions from the fibers, which was depended on the moisture absorption of the matrix, was an important parameter to affect the antibacterial efficiency.