Paper Title:
Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector
  Abstract

Silkworm BmN4 cells were transfected with the helper plasmid and the piggyBac vector( piggyBac-FLAG) in which was inserted with the spider flagelliform silk expression cassette. Via antibiotic selection, most cells showed stable DsRed-expression. Immuno blot analysis showed that flagelliform silk protein of spider was expressed stably in BmN4 cells. Circular dichroism spectra indicated the existence of β-turn structure in recombinant spider flagelliform protein. The present results suggested that transgenic silkworm directly secreting functional spider silk protein in cocoon by using piggyBac system is feasible.

  Info
Periodical
Advanced Materials Research (Volumes 332-334)
Chapter
Chapter 5: Structure, Properties and Processes of Textile Materials
Edited by
Xiaoming Qian and Huawu Liu
Pages
779-782
DOI
10.4028/www.scientific.net/AMR.332-334.779
Citation
Y. S. Zhang, T. F. Zhao, A. C. Zhao, M. Nakagaki, "Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector", Advanced Materials Research, Vols. 332-334, pp. 779-782, 2011
Online since
September 2011
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Price
$32.00
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