Though polyester fabric has favorable characteristics, it is not comfortable to wear because of its hydrophobic characteristics Silk fibroin has lots of polar and electric groups, and its biocompatible is very good. In this paper polyester fabric was encased sucrose ester to endue polyester fibers lots of –OH, then sucrose eater glycidyl ether (SEGE) which not only had crosslink function but also had softening function was used to graft silk fibroin on polyester fabric. FT-IR spectra showed silk fibroin was grafted on polyester fabric; DSC showed thermostability of fabric kept very well; X-ray diffraction spectra showed the structure of some silk fibroin grafted on polyester fabric was -sheet. With the increase of grafting rate of silk fibroin on polyester fabric, the broken strength of polyester fabric grafted silk fibroin did not decrease; cockle elastics kept very well; antistatic property and moisture regain increased greatly, whiteness and air permeability kept very well and fabric was still soft enough. The polyester fabric grafted silk fibroin was high functional.