To study whether the architectural parameters are sensitive to the shape of volume of interests in micro-CT scanning, six human C5 body samples were scanned by micro-CT. Cubic and cylindrical volumes of interests were acquired as the length of cubic samples was being changed continually. The nonlinear curve fitting method was employed to explore the correlation between the parameters and the volume of interests. The selected morphological indices showed a significantly variable tendency for the lengths of cubic and cylindrical regions of interests, except the Tb.Th and BS/BV. The Dunnett-t tests were performed to compare the architectural parameters of different region of interests against that of control group. There was no significant difference observed between the architectural parameters from cubic region of interests and that from the cylindrical region of interests.